Journal: Nutrients

Article Title: Essential Oils, Pituranthos chloranthus and Teucrium ramosissimum , Chemosensitize Resistant Human Uterine Sarcoma MES-SA/Dx5 Cells to Doxorubicin by Inducing Apoptosis and Targeting P-Glycoprotein

doi: 10.3390/nu13051719

Figure Lengend Snippet: Effects of Pituranthos chloranthus (PC) and Teucrium ramosissimum Desf. (TR) extracts on normal primary human uterine fibroblast cells (HUF) and primary murine Bone Marrow-Derived Macrophages (BMDM) viability. After treatment of primary HUF and murine BMDM with increasing concentrations (0–100 µg/mL) of PC and TR for 72 h, the percentage of viable cells was assessed using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. ( A ) Dose–response curves of PC-treated HUF (left panel) and TR-treated HUF (right panel). ( B ) Dose–response curves of PC-treated BMDM (left panel) and TR-treated BMDM (right panel). Data are expressed as a mean percentage of control growth ± Standard Deviation (SD) of two representative experiments ( n = 6 replicates per concentration).

Article Snippet: Primary human uterine fibroblast normal cells (HUF) were obtained from the ATCC.

Techniques: Derivative Assay, MTT Assay, Control, Standard Deviation, Concentration Assay

Journal: The Journal of investigative dermatology

Article Title: Wavelength-specific synergy between ultraviolet radiation and interleukin-1 alpha in the regulation of matrix-related genes: mechanistic role for tumor necrosis factor-alpha.

doi: 10.1046/j.1523-1747.1999.00681.x

Figure Lengend Snippet: Figure 1. Effects of specific wavelengths on the ability of IL-1α to stimulate matrix-related mRNA in fibroblasts. Human cultured fibroblasts were irradiated with 30 mJ of UVB, 5 J of UVA, 5 J of UVA1, or sham irradiated, and then given media without (minus symbols) or with (plus symbols) 10 ng IL-1α per ml, followed by 24 h of incubation at 37°C. Displayed are normalized levels of tropoelastin (A) and collagenase (B) mRNA.

Article Snippet: Cultured cells Normal human fibroblasts were obtained from the American Type Culture Collection (ATCC, Rockville, MD, catalog no. 1828X-CRL) and grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum.

Techniques: Cell Culture, Irradiation, Incubation

Journal: The Journal of investigative dermatology

Article Title: Wavelength-specific synergy between ultraviolet radiation and interleukin-1 alpha in the regulation of matrix-related genes: mechanistic role for tumor necrosis factor-alpha.

doi: 10.1046/j.1523-1747.1999.00681.x

Figure Lengend Snippet: Figure 2. Effects of specific wavelengths on cellular secretion of TNF-α. (a) Fibroblasts were irradiated with 30 mJ of UVB, 5 J of UVA, 5 J of UVA1, or sham irradiated, and then given media without (minus symbols) or with (plus symbols) 10 ng IL-1α per ml, followed by 24 h of incubation at 37°C. Displayed are TNF-α concentrations in the conditioned media (n 5 2). (b) Keratinocytes were similarly irradiated, incubated, and media assayed for TNF-α. Displayed are TNF-α concentrations in the conditioned media when no exogenous IL-1α had been added (n 5 2). Separate experiments demonstrated that exogenous IL-1α did not affect secretion of TNF-α by keratinocytes exposed to any of these wavelengths. (c) Neonatal and adult keratinocytes were irradiated with 10 or 30 mJ per cm2 of UVB or 5 or 20 J per cm2 of UVA. Displayed are TNF-α concentrations in the conditioned media from irradiated neonatal (n 5 2) and adult (n 5 3) keratinocytes, p , 0.01 for adult 30 mJ per cm2 UVB relative to sham by analysis of variance, then Dunnett q9 statistic.

Article Snippet: Cultured cells Normal human fibroblasts were obtained from the American Type Culture Collection (ATCC, Rockville, MD, catalog no. 1828X-CRL) and grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum.

Techniques: Irradiation, Incubation

Journal: The Journal of investigative dermatology

Article Title: Wavelength-specific synergy between ultraviolet radiation and interleukin-1 alpha in the regulation of matrix-related genes: mechanistic role for tumor necrosis factor-alpha.

doi: 10.1046/j.1523-1747.1999.00681.x

Figure Lengend Snippet: Figure 3. Effects of exogenous TNF-α on the ability of IL-1α to stimulate tropoelastin mRNA in fibroblasts. Human cultured fibroblasts were incubated with or without IL-1α and TNF-α, as indicated. Displayed are normalized tropoelastin mRNA levels after 24 h of incubation at 37°C. IL-1α produced a statistically significant increase in tropoelastin mRNA only in the cells that received no TNF-α (p , 0.01 by t test).

Article Snippet: Cultured cells Normal human fibroblasts were obtained from the American Type Culture Collection (ATCC, Rockville, MD, catalog no. 1828X-CRL) and grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum.

Techniques: Cell Culture, Incubation, Produced

Journal: The Journal of investigative dermatology

Article Title: Wavelength-specific synergy between ultraviolet radiation and interleukin-1 alpha in the regulation of matrix-related genes: mechanistic role for tumor necrosis factor-alpha.

doi: 10.1046/j.1523-1747.1999.00681.x

Figure Lengend Snippet: Figure 4. Effect of anti-TNF-α antibodies on the ability of UVB to block the effect of IL-1α on matrix-related messages. Human cultured fibroblasts were irradiated with 30 mJ of UVB or sham irradiation, and then given media without (minus symbols) or with (plus symbols) 10 ng IL-1α per ml, plus the indicated concentrations of anti-TNF-α inhibitory antibodies. Displayed are normalized tropoelastin (a) and collagen (b) mRNA levels after 24 h of incubation at 37°C.

Article Snippet: Cultured cells Normal human fibroblasts were obtained from the American Type Culture Collection (ATCC, Rockville, MD, catalog no. 1828X-CRL) and grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum.

Techniques: Blocking Assay, Cell Culture, Irradiation, Incubation

Journal: Journal of Biological Chemistry

Article Title: Evidence That Factor VIII Forms a Bivalent Complex with the Low Density Lipoprotein (LDL) Receptor-related Protein 1 (LRP1)

doi: 10.1074/jbc.m116.754622

Figure Lengend Snippet: FIGURE 7. Effect of clusters II and IV on LRP1-mediated internalization of BDD-fVIII (A) and tPAPAI-1 (B) by WI-38 fibroblasts. WI-38 fibroblasts were seeded in 12-well tissue culture dishes (1 105 cells/well) and incubated with 20 nM 125I-BDD-fVIII (A) or 10 nM 125I-tPAPAI-1 (B) for 4 h at 37 °C in the presence orabsenceofLRP1clusterIIorIV(500nM).Followingincubation,theamountofradiolabeledligandinternalizedwasdetermined,andthedatawerenormalized to the amount of fVIII internalized in the absence of competitors. The results represent the mean of three independent experiments. Error bars represent S.E. (*, p 0.002, one-way analysis of variance with Tukey’s multiple comparison post-test).

Article Snippet: WI38 cells, human lung fibroblast cells, were obtained from American Type Culture Collection (ATCC) and maintained in DMEM (Corning) supplemented with 10% FBS and penicillin/streptomycin.

Techniques: Incubation, Comparison